![]() ![]() By the use of phenotype and complementation tests, the 98 fs lines were resolved into 19 fs genes on the 2nd chromosome and 17 fs genes on the 3rd chromosome. The protocol can easily be adapted to different affinity-tagged or endogenous protein complexes of interest.Ī newly derived collection of 98 autosomal recessive female-sterile (fs) lines induced by ethyl methanesulfonate (EMS) in Drosophila melanogaster has been studied genetically and cytologically. The procedure can be subdivided into the following steps: acquisition of sufficient starting material, complex stabilization by crosslinking (optional), purification of the protein complex by immunoprecipitation, separation of the isolated material on a polyacrylamide gel, sample preparation for mass spectrometry, and sample analysis. Here, we present a basic method for the purification of the endogenous Par-6/aPKC protein complex, which plays a central role in orchestrating asymmetric cell divisions in the developing nervous system of Drosophila. ![]() Therefore, Drosophila now offers the advantages of both genetic and biochemical approaches. Protein biochemical methods have lagged behind for quite some time but meanwhile have reached a state where protein interaction networks can be elucidated at a similar speed and accuracy as genetic interactions. Drosophila melanogaster is one of the best characterized model systems for genetic analysis.
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